Time-resolved fluorescence spectroscopy

• Fluorescence decay curves
  • Fluorescence decay curve reading
  • Fluorescence decay curve attributes
  • Operations on fluorescence decays
  • Integrating data
  • FRET induced donor decay
  • Time-resolved anisotropy
• Fluorescence decay forward model
  • Data reading
  • Fluorescence decay forward model
  • Scoring
  • Scale to data
  • Score
• Fluorescence decay objective function
• Fluorescence decay analysis in ChiSurf
  • Time-correlated single photon counting: Basics
  • Loading data in ChiSurf
  • FRET sensitized emission